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This Concept Map, created with IHMC CmapTools, has information related to: Lecture Notes 9.06 Chapter 3&4, proto oncogene lead to the belief that regulation of tumor formation, incapacitating a protein i.e temperature sensative mutants, proto oncogene is transformed into oncogene, retrovirus uses reverse transcription, Small Genome Viruses can help biologists understand transformed cells, transfection can be transcient, regulation of tumor formation not wedded out by evoultion, reverse transcription allowed for other techniques, non cancerous cells know as proto oncogene, transfection works best with 3T3 cells, cancerous cells known as oncogene, transfection can occur betweeen species, a Southern Blot amplified by PCR, oncogene can integrate into a genome, transcient where the plasmid does not integrate into the genome, small DNA fragments of viral DNA aka cDNA, calcium phosphat transfection is a common type of transfection, plasmid integrates intot he genome by crossover, evoultion therefore it is a vital function, Src Gene found in cancerous cells