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This Concept Map, created with IHMC CmapTools, has information related to: group discussion sept 6 ch 4, gene conservation eg protein from gene stays conserved, mutations may be small and subtle, gene conservation is disrupted by mutations, virus related cancer found in large numbers in animals, gene is normal, signal got sent improperly can be affected by mutations, using a virus to pass the DNA causes stable transfection, virus related cancer occur sparsely in humans, use 3T3 cells -> susceptible to transfection more was thought of as stacking the deck, stacking the deck because cell type are too eager to transfect, isoforms eg one gene, x variations on a protein, growth receptor protein mutated by oncogene does not allow for ligand binding, transfection from cancer cells to normal cells was an important method because DNA transfer caused cancer in previously healthy cell, stable transfection must retain ability to be transcripted and translated, transfection from cancer cells to normal cells was accomplished through isolate plasmid of interest, isoforms are very prevalent, NIH heavily funded research about virus related cancer, splicing performed by spliceozyme, viral genome integration could be located using antibodies to detect viral proteins, transfection from one species to another is important due to possible technical ease, structure stays conserved is important because small changes cause misfolding