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This Concept Map, created with IHMC CmapTools, has information related to: Chapters 3 and 4, reverse transcriptase to carry out viral reproduction, RSV tumor contains single-stranded RNA, viral oncogene e.g. v-src, transfection is done using transient transfection, src gene contributes to cell growth, src gene has forms c-src, Howard Temin discovered reverse transcriptase, transformed DNA contains oncogene, temperature-sensitive mutants of viral oncogene, viral reproduction in host cells, c-src underwent a mutation, temperature-sensitive mutants as genes, restrictive temperature to analyze normal function, genes may be turned on or off using restrictive temperature, oncoprotein can have serveral isoforms, permissive temperature to analyze normal function, isoforms using mechanism of alternative splicing, permissive temperature to analyze mutated function, transfection and then form animal tumors, alternative splicing that is carried out by a spliceosome