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This Concept Map, created with IHMC CmapTools, has information related to: Histological Methods, Frozen in OCT, Chemically Fixed preserves Microstructure, Frozen is Fast, Chemically Fixed in Acid, Embedding requires Dehydration, Histotechnique prepares Tissue samples, Cryostat preserves Lipids, Plastic polymerizes to Harden, Freeze-substitution for SEM, Sectioning on Cryostat, Microtome to make Sections, Chemically Fixed in Glutaraldehyde, Frozen dehydrated Freeze-substitution, Tissue samples can be Frozen, Ultramicrotome used in Electron Microscopy, Chemically Fixed in Alcohol, Slicing with a Microtome, Ethanol miscible with Clearing Agent, Plastic resins including Methyl methacrylate, Paraffin wax used for Light Microscopy