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This Concept Map, created with IHMC CmapTools, has information related to: Bacterial Identification, a patient sample from the sample we Grow Bacterial Cultures, a patient sample or stool, Bacterial Identification First: requires a patient sample, PCR (Polymerase Chain Reaction) Third: after PCR we Purify the target DNA Sequence, Bacterial Identification relies on DNA Sequencing, DNA Sequencing in order to sequence we need DNA Amplification (make many copies of the target sequence), Compare to Known DNA Sequences from a Database Finally: based on comparison we can Identify the Bacterium causing the reported symptoms, a patient sample such as saliva, Purify the target DNA Sequence Fourth: we use a machine to Sequence the DNA Sample, Sequence the DNA Sample Fifth: we can Compare to Known DNA Sequences from a Database, Grow Bacterial Cultures and Isolate the Bacterial DNA, DNA Amplification (make many copies of the target sequence) for this we use PCR (Polymerase Chain Reaction), Isolate the Bacterial DNA Second: we use PCR (Polymerase Chain Reaction), Purify the target DNA Sequence this allows us to Compare to Known DNA Sequences from a Database, Bacterial Identification try this one: http://www.hhmi.org/biointeractive/vlabs/bacterial_id/index.html, a patient sample or blood