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This Concept Map, created with IHMC CmapTools, has information related to: Biotechnology Plant, Broth Tank Broth Centrifuge, ruptured cells Homogenizer (Cell Disruptor) [2nd time], Recovery clarified lysate in transfer vessel, lysate .22 Micron Filter (remove the solids), High Salt Eluate Tangential-Flow Filtration (Ultrafiltration Membrane) Ultrafiltration (concentration, pH Sensor ++Beads in the Chromatography Column, Aseptic Hood Broth Bioreactor, Centrifuge cleaned cell paste suspended cleaned cells, NaCl Solution to Release GFP from Beads eluate (product) UV Optical Sensor, Centrifuge clarified stream Discard the Liquid, ++Beads in the Chromatography Column elution NaCl Solution to Release GFP from Beads, Recovery Purification, Freezer Seed Stock Aseptic Hood, Ammonium Sulfate Permanent (Waste) Retentate (Product), Bioreactor Broth Broth Tank, Concentrate GFP High Salt Eluate Pump to next Process, Suspended Cleaned Cells Homogenizer (Cell Disruptor) [2nd time], Homogenizer (Cell Disruptor) [2nd time] ruptured cells ruptured cells, .22 Micron Filter (remove the solids) clarified lysate (biological soup) Lysate Transfer Vessel, Column Chromotography (for Diafilration) Non GFP solution