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This Concept Map, created with IHMC CmapTools, has information related to: Biotechnology Facility, Concentrate GFP High Salt Eluate Pressure Sensor, Purification Classified Lysate in Trnsfer Vessel Pressure sensor, Recovery Lysate 0.22 Micron Filter (remove the solids) Bacteria is 1 micron in diameter, Purification Classified Lysate in Trnsfer Vessel Flow meter, ++Beads in the Chromatography Column Non GFP solution is waste pH Sensor, Recycle until concentrated Second TFF process New Buffer, Homogenizer Ruptured Cells Discard the solid, Fermentation Seed stock Freezer, Air meter Filtered Clarified Lysate (pH 8.0) Column Chromatography for Diafiltration, pH Sensor Elution NaCl Solution to Release GFP from Beads, Purification Classified Lysate in Trnsfer Vessel 0.45 Micron Pre-filter, Concentrate GFP High Salt Eluate Ultrafiltration, Cell Paste (Solid) Suspended Cells in Cleaning solution Centrifuge, Concentrate GFP High Salt Eluate Ammonium Sulfare, Eluate Collection Vessel Concentrate GFP, Purification Classified Lysate in Trnsfer Vessel Air meter, Purification Classified Lysate in Trnsfer Vessel Conductivity Sensor, Concentrate GFP High Salt Eluate Tangential-Flow Filtration, Cleaned Cell Paste Suspended Clean Cells Homogenizer, Eluate Checking for GFP UV Optical Sensor